By Doolittle E.
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Extra info for A Study of the Motions of Forty-eight Double Stars (1917)(en)(3s)
These constants are a function of both the polymer and its solvent environment (including temperature). If the constants are available from the literature or can be determined for the sample polymer using narrow fractions in the SEC mobile phase, then one can substitute the Mark – Houwink term for [h] into Eq. (18) to yield log10 M2 ¼ 1 K1 1 þ a1 log10 þ log10 M1 1 þ a2 K2 1 þ a2 (20) which is an expression for the sample molecular weight in terms of the standard molecular weight and both sets of Mark – Houwink constants.
6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. REFERENCES J Porath, P Flodin. Nature 183:1657, 1959. JC Moore. J Polym Sci, Part A 2:835, 1964. B Ravindranath. Principles and Practice of Chromatography. Ellis Horwood Ltd, UK, 1989, p 317. PA Bristow. Liquid Chromatography in Practise. UK: Hept, 1976, p 16. AB Littlewood. Gas Chromatography. New York: Academic Press, 1970. C Wu. Column Handbook for Size Exclusion Chromatography. New York: Academic Press, 1999. J Seidl, J Malinsky, K Dusek, W Heitz.
Since their introduction in 1978, high-performance silica-based SEC packings have made a great impact in the analysis and puriﬁcation of biopolymers. Columns ﬁlled with 10-mm spherical particles and nominal pore sizes of 125, 250, ˚ (10 A ˚ ¼ 1 nm) became the state of the art for protein separations and 500 A during the 1980s (6). Further improvements in speed and resolution were obtained by reducing the size of the particles from 10 to 5 mm (7). Columns ﬁlled with these high-performance particles are now manufactured and distributed by several companies.
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